rnascope probe dapb Search Results


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rnascope® negative control probe- dapb  (Advanced Cell Diagnostics Inc)
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bacterial rnascope dapb probe  (Advanced Cell Diagnostics Inc)
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2018 other dapi stain molecular probes  (Advanced Cell Diagnostics Inc)
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Figure 2. Expression of at least one CMF gene is seen in ~59% of cardiac ECs. (A): Analysis of mouse heart and lung single cell data from Tabula Muris in Schaum, et al (Nature, <t>2018).</t> Fifteen cardiac myofibrillar (CMF) genes were chosen based on high, unique expression in the cardiomyocyte cell subsets. Positive expression was determined as a normalized count (ln(1+Counts-per-million)) greater than or equal to one. (B): Percentage of cardiomyocytes vs endothelial cells or fibroblasts within the heart and lung that express 1–15 of the selected CMF genes. (C–E). Analysis of unspliced vs spliced transcripts in Tabula Muris data. Data shown are merged counts for all cells within the cardiomyocyte (CM), endothelial cell (EC), or fibroblast Figure 2 continued on next page
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negative control sirna  (Bio-Techne corporation)
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Figure 2. Expression of at least one CMF gene is seen in ~59% of cardiac ECs. (A): Analysis of mouse heart and lung single cell data from Tabula Muris in Schaum, et al (Nature, <t>2018).</t> Fifteen cardiac myofibrillar (CMF) genes were chosen based on high, unique expression in the cardiomyocyte cell subsets. Positive expression was determined as a normalized count (ln(1+Counts-per-million)) greater than or equal to one. (B): Percentage of cardiomyocytes vs endothelial cells or fibroblasts within the heart and lung that express 1–15 of the selected CMF genes. (C–E). Analysis of unspliced vs spliced transcripts in Tabula Muris data. Data shown are merged counts for all cells within the cardiomyocyte (CM), endothelial cell (EC), or fibroblast Figure 2 continued on next page
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dapi  (Millipore)
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Figure 2. Expression of at least one CMF gene is seen in ~59% of cardiac ECs. (A): Analysis of mouse heart and lung single cell data from Tabula Muris in Schaum, et al (Nature, <t>2018).</t> Fifteen cardiac myofibrillar (CMF) genes were chosen based on high, unique expression in the cardiomyocyte cell subsets. Positive expression was determined as a normalized count (ln(1+Counts-per-million)) greater than or equal to one. (B): Percentage of cardiomyocytes vs endothelial cells or fibroblasts within the heart and lung that express 1–15 of the selected CMF genes. (C–E). Analysis of unspliced vs spliced transcripts in Tabula Muris data. Data shown are merged counts for all cells within the cardiomyocyte (CM), endothelial cell (EC), or fibroblast Figure 2 continued on next page
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dapb probe  (Advanced Cell Diagnostics Inc)
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Figure 2. Expression of at least one CMF gene is seen in ~59% of cardiac ECs. (A): Analysis of mouse heart and lung single cell data from Tabula Muris in Schaum, et al (Nature, <t>2018).</t> Fifteen cardiac myofibrillar (CMF) genes were chosen based on high, unique expression in the cardiomyocyte cell subsets. Positive expression was determined as a normalized count (ln(1+Counts-per-million)) greater than or equal to one. (B): Percentage of cardiomyocytes vs endothelial cells or fibroblasts within the heart and lung that express 1–15 of the selected CMF genes. (C–E). Analysis of unspliced vs spliced transcripts in Tabula Muris data. Data shown are merged counts for all cells within the cardiomyocyte (CM), endothelial cell (EC), or fibroblast Figure 2 continued on next page
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Image Search Results


Figure 2. Expression of at least one CMF gene is seen in ~59% of cardiac ECs. (A): Analysis of mouse heart and lung single cell data from Tabula Muris in Schaum, et al (Nature, 2018). Fifteen cardiac myofibrillar (CMF) genes were chosen based on high, unique expression in the cardiomyocyte cell subsets. Positive expression was determined as a normalized count (ln(1+Counts-per-million)) greater than or equal to one. (B): Percentage of cardiomyocytes vs endothelial cells or fibroblasts within the heart and lung that express 1–15 of the selected CMF genes. (C–E). Analysis of unspliced vs spliced transcripts in Tabula Muris data. Data shown are merged counts for all cells within the cardiomyocyte (CM), endothelial cell (EC), or fibroblast Figure 2 continued on next page

Journal: eLife

Article Title: Cardiac endothelial cells maintain open chromatin and expression of cardiomyocyte myofibrillar genes

doi: 10.7554/elife.55730

Figure Lengend Snippet: Figure 2. Expression of at least one CMF gene is seen in ~59% of cardiac ECs. (A): Analysis of mouse heart and lung single cell data from Tabula Muris in Schaum, et al (Nature, 2018). Fifteen cardiac myofibrillar (CMF) genes were chosen based on high, unique expression in the cardiomyocyte cell subsets. Positive expression was determined as a normalized count (ln(1+Counts-per-million)) greater than or equal to one. (B): Percentage of cardiomyocytes vs endothelial cells or fibroblasts within the heart and lung that express 1–15 of the selected CMF genes. (C–E). Analysis of unspliced vs spliced transcripts in Tabula Muris data. Data shown are merged counts for all cells within the cardiomyocyte (CM), endothelial cell (EC), or fibroblast Figure 2 continued on next page

Article Snippet: DOI: https://doi.org/10.7554/eLife.55730 12 of 19 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Software, algorithm Genrich https://github.com/jsh58/Genrich Software, algorithm Picard Tools http://broadinstitute.github.io/picard/ Software, algorithm samtools Li et al., 2009 Software, algorithm STAR Dobin, 2013 Software, algorithm Seurat Stuart et al., 2019 Software, algorithm R https://www.r-project.org/ Software, algorithm DiffBind Ross-Innes et al., 2012 Software, algorithm Deeptools Ramı́rez et al., 2016 Software, algorithm Homer Heinz et al., 2010 Software, algorithm Velocyto La Manno et al., 2018 Other DAPI stain Molecular Probes (1:1000) Other RNAscope Probe- MmTnnt2-C3 ACD Bio Cat# 418681-C3 Other RNAscope Probe- MmCdh5-C2 ACDBio Cat# 312531-C2 Commercial assay or kit RNAscope Multiplex Fluorescent v2 Assay ACD Bio Cat# 323136 Commercial assay or kit Illumina Tagment DNA Enzyme and Buffer Illumina Cat#:20034197 1.25 uL of enzyme used per 50,000 nuclei for transposition

Techniques: Expressing